Published April 18, 2008; doi:10. 0363-6135/08 $8.Versions of this Article: 294/6/H2712 _most recent_ Services Google Scholar PubMed ANGIOTENSIN II INCREASES GABAB RECEPTOR EXPRESSION IN NUCLEUS TRACTUS SOLITARII OF RATS FANRONG YAO,1 COLIN SUMNERS,2 STEPHEN T.AND CHENGWEN SUN1 _ 1Department of Pharmaceutical Sciences, College of Pharmacy, North Dakota State University, Fargo, North Dakota; and 2Department of Physiology and Functional Genomics, College of Medicine, University of Florida, Gainesville, Florida _ Submitted 22 June 2007 ; accepted in final form 15 April 2008 Increasing evidence indicates that both the angiotensin II (ANG II) and -aminobutyric acid (GABA) systems play a very important role in the regulation of blood pressure (BP).There is little information concerning the interactions between these two systems in the nucleus tractus solitarii (NTS).The present study, we examined the effects of ANG II on GABAA and GABAB receptor (GAR and GBR) expression in the NTS of Sprague-Dawley rats.Direct effect of ANG II on GBR expression was determined in neurons cultured from NTS.Neuronal cultures with ANG II (100 nM, 5 h) induced a twofold increase in GBR1 expression, as detected with real-time RT-PCR and Western blots, but had no effect on GBR2 or GAR expression.Experiments, perfusion of neuronal cultures with the GBR agonist baclofen decreased neuronal firing rate by 39% and 63% in neurons treated with either PBS (control) or ANG II, respectively, indicating that chronic ANG II treatment significantly enhanced the neuronal response to GBR activation.Contrast, ANG II had no significant effect on the inhibitory action of the GAR agonist muscimol.Whole animal studies, intracerebroventricular infusion of ANG II induced a sustained increase in mean BP and an elevation of GBR1 mRNA and protein levels in the NTS.Results indicate that ANG II stimulates GBR expression in NTS neurons, and this could contribute to the central nervous system actions of ANG II that result in dampening of baroreflexes and elevated BP in the central actions of ANG II.-aminobutyric acid; blood pressure Address for reprint requests and other correspondence: C.Dept.North Dakota State Univ.Read more [mechanisms of signal transduction] regulation of interleukin-6-induced hepatic insulin resistance by mammalian target of rapamycin through the stat3-socs3 pathway
Get more Hoxa9 induces insulin-like growth factor-1 receptor expression in b-lineage acute lymphoblastic leukemia
