March 8, 2007; doi:10. 0193-1857/07 $8.All Versions of this Article: 292/6/G1695 _most recent_ Services Citing Articles Google Scholar PubMed LIVER AND BILIARY TRACT MEASUREMENT OF LIVER COLLAGEN SYNTHESIS BY HEAVY WATER LABELING: EFFECTS OF PROFIBROTIC TOXICANTS AND ANTIFIBROTIC INTERVENTIONS JAMES L.M.ABRAHAM BAUTISTA,1 GLEN LINDWALL,1 MOHAMAD AWADA,1 AND MARC K._ 1KineMed, Inc.2Department of Nutritional Sciences and Toxicology, University of California, Berkeley; and 3Department of Medicine, San Francisco General Hospital, University of California, San Francisco, California _ Submitted 12 May 2006 ; accepted in final form 2 February 2007 Enhanced production of collagen is central to fibrotic disorders such as hepatic cirrhosis and pulmonary fibrosis.Describe a sensitive, quantitative, and high-throughput technique for measuring hepatic collagen synthesis in vivo through metabolic labeling with heavy water (2H2O).And mice received 2H2O in drinking water for up to 35 days.Into collagen-bound amino acids (AA) alanine and hydroxyproline (OHP) was viagra on line measured by gas chromatography-mass spectrometry.Threefold stimulation of collagen fractional synthesis was observed under the maximum dosage of carbon tetrachloride (CCl4; 1.Deuterium enrichment was systematically 20% higher in AA from monomeric collagen relative to dimeric collagen, consistent with slower turnover of the latter.1% griseofulvin to mice resulted in a significant, threefold increase in liver book casino guest viagra collagen synthesis, observable within 12 days and consistent with predicted interstrain differences (C57/Bl6J > BALB/c).Enrichments of OHP from total liver proteins correlated well 6 free viagra with alanine or OHP from isolated collagen.After withdrawal of CCl4 exposure and was reduced to various degrees by coadministration free viagra sample of interferon-, rosiglitazone, atorvastatin, or enalapril.In isotopically measured collagen synthesis correlated with, but were more sensitive and reproducible than, standard histological staining (trichrome) for fibrosis.Liver collagen synthesis can be measured sensitively and with high precision over a short time chinese herbal viagra period, without radioactivity, thereby providing a relatively high-throughput in vivo strategy for rapidly measuring profibrotic activities of suspected hepatotoxicants online prescription scams viagra and antifibrotic activities of drug candidates.Fibrogenesis; hydroxyproline; in vivo; strain differences Address for reprint requests and other correspondence: M.Dept.
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