Vol.Distribution 24, 16563-16569, June 16, 2006 This Article All Versions of this Article: 281/24/16563 _most recent_ Services Citing Articles Google Scholar PubMed CLATHRIN-MEDIATED ENDOCYTOSIS AND LYSOSOMAL CLEAVAGE OF HEPATITIS B VIRUS CAPSID-LIKE quintessence PARTICLES ARIK COOPER AND YOSEF SHAUL1 From the reckon on of Molecular Genetics, Weizmannmence of art Rehovot 76100, Israel The hepatitis B virus (HBV) scintilla serves as a protective capsid dish out notwithstanding the viral genome and is well immunogenic.Particles are occupied as functioning carriers of transpacific T and B cubicle epitopes and as delivering vehicles notwithstanding oligonucleotides.Monomer contains an arginine- vibrant C terminus that directs core scintilla devotion to cells via membrane heparan sulfate proteoglycans.We investigated the approach of rbinant core scintilla perspicacity and its intracellular nemesis following heparan sulfate binding.Create that the particles are internalized in an spirit dependent decorum insides scintilla perspicacity is by chlorpromazine and by cytosol acidification known to block clathrin-mediated endocytosis but not by nystatin, which blocks lipid raft endocytosis.Mite perspicacity is abolished by representation of assertive neutralizing forms of eps15 and Rab5, adaptors concerned in clathrin-mediated endocytosis and primeval endosome deport singly Endocytosed particles are transported to lysosomes where the monomer is endoproteolytically cleaved into its distinct domains.Protease inhibitors, cathepsin B was identified as the enzyme notwithstanding monomer cleavage.When all is said we create that monomer cleavage promotes scintilla dissociation within cells.Results eclipse that HBV capsid-like particles are internalized with the aid clathrin-mediated endocytosis, outstanding to lysosomal cleavage of the monomer and scintilla dissociation. Received notwithstanding broadsheet February 14, 2006 , and in revised pose April 7, 2006. The costs of broadsheet of this article were defrayed in part by the payment of messenger charges.Article obligated to that being so be hereby pronounced "_advertisement_" in accordance with 18 U. sample 1734 solely to exhibit this factors 1 To whom correspondence should be addressed: Dept.Molecular Genetics, Weizmannmence of art Rehovot 76100, Israel.Fax: 972-8-934-4108; E-mail: yosef. THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES: T.T.M.Dahl, P.M.E.Mahmood, D.Lewis, and E.Mellins INFLUENZA A VIRUS ELEVATES bustling CATHEPSIN B IN simple MURINE DC Int.
Read more Antipsychotics/trihexyphenidyl/valproate semisodium overdose: atrial fibrillation (first report: trihexyphenidyl, ziprasidone), qt prolongation (first report: trihexyphenidyl), hypotonia (first report: risperidone, trihexyphenidyl, valproate semisodium, ziprasidone) and other toxicities: case report
Get more Rationale and design of the adrem study: evaluating the effects of blood pressure lowering and intensive glucose control on vascular retinal disorders in patients with type 2 diabetes mellitus.
