Published November 12, 2007; 10. This Article All Versions of this Article: 54/1/116 _most recent_ Submit an electronic Letter to the Editor about this paper Services Google Scholar PubMed (_Clinical Chemistry.(c) 2008 American Association for Clinical Chemistry, Inc. AUTOMATION AND ANALYTICAL TECHNIQUES NANOGOLD CATALYSIS-BASED IMMUNORESONANCE-SCATTERING SPECTRAL ASSAY FOR TRACEPLEMENTPONENT 3 ZHILIANG JIANG1,2,A, WENXING HUANG2, JIANGPING LI1, MINGSHUN LI2, AIHUI LIANG1, SHENGSEN ZHANG2 AND BING CHEN2 1 Key Laboratory of New Processing Technology for Nonferrous Metals and Materials of Education Ministry, Department of Material and Chemical Engineering, Guilin University of Technology, Guilin, China, 2 School of Environment and Resource, Guangxi Normal University, Guilin, China.Correspondence to this author at: Zhiliang Jiang, School of Environment and Resource, Guangxi Normal University, Guilin 541004, China.+86-0773-5846201; e-mail zljiang{at}mailbox.Or zljiang{at}glite. Background:plementponent 3 (C3) is an essential bridge linking innate immunity and adaptive immunity.Describe an immunonanogold catalytic resonance-scattering (RS) technique for assaying C3 in serum.Methods: We used nanogold to label goat antihuman C3 antibody to obtain an immunonanogold RS probe for C3.Reaction between nanogold-labeled antibodies and antigens was carried out in Na2HPO4-sodium citrate buffer, pH 5.Glycol.The particle suspension, we used RS to monitor the catalytic effect of nanogold-labeled anti-C3 in the supernatant on the chlorauric acid-hydroxylamine (HAuCl4-NH2OH) particle reaction and used electron microscopy to monitor particle shape.Assayed 36 human serum samples with the immunonanogold catalytic RS assay and immunoturbidimetry.Results: Nanogold-labeled anti-C3 had a marked catalytic effect on the reaction of HAuCl4 and NH2OH to form particles, which exhibit a maximum RS peak at 585 nm.In RS intensity, IRS, of the nanocatalytic system was proportional to C3 concentration from 5.160.The detection limit for the C3 assay was 1.Obtained with serum samples agreed with those obtained with an immunoturbidimetric method.Linear regression analysis of 28 nonpathologic serum samples revealed a correlation coefficient of 0.Mean (SD) slope and intercept values of 0.G/L and 0.G/L C3, respectively.Read more Ramipril, rosiglitazone for atherosclerosis: starr results?
Get more Original investigation: the variability and quality of medication container labels
