Or contact us to report problems with: For Publishers For Researchers For Librarians FIRST-GENERATION ANTIHISTAMINES DIPHENHYDRAMINE AND CHLORPHENIRAMINE REVERSE CYTOKINE-AFFORDED EOSINOPHIL SURVIVAL BY ENHANCING APOPTOSIS AUTHORS: Hasala, Hannele; Moilanen, Eeva; Janka-Junttila, Mirkka; Giembycz, Mark A.Hannu SOURCE: , Volume 28, Number 1, January-February 2007 , pp.PUBLISHER: Key: - Free Content - New Content - Subscribed Content - Free Trial Content ABSTRACT: Antihistamines or histamine H1-receptor antagonists aremonly used to treat a variety of allergic symptoms.Are considered to play an essential role in the pathogenesis of allergy.Apoptosis is thought to be an important element in the formation of eosinophilia in allergic conditions such as allergic rhinitis, atopic eczema, and asthma.Of this study was to investigate the effects of two first-generation antihistamines diphenhydramine and chlorpheniramine on constitutive eosinophil apoptosis and on interleukin (IL)-5-afforded eosinophil survival.Role of c-Jun N-terminal kinase (JNK) in mediating the effects of antihistamines on eosinophil apoptosis was evaluated also.Of isolated human eosinophils was assessed by measuring the relative DNA content of propidium iodide-stained cells and confirmed by morphological analysis.Of JNK was measured by Western blotting.Were found to reverse the survival-prolonging effect of IL-5 in eosinophils by enhancing apoptosis.Was found to be activated slowly during diphenhydramine-induced eosinophil apoptosis.Inhibitor peptide specific for JNK, L-JNKI1 (JNK peptide inhibitor 1, L-stereoisomer), inhibited diphenhydramine-mediated eosinophil apoptosis.Results suggest that first-generation antihistamines diphenhydramine and chlorpheniramine reverse IL-5-afforded eosinophil survival and that the enhanced apoptosis by antihistamines is mediated through activation of JNK.Reversal of IL-5-afforded eosinophil survival may contribute to the antiallergic actions of diphenhydramine and chlorpheniramine.KEYWORDS: ; ; ; ; DOCUMENT TYPE: Research article DOI: 10.2961 AFFILIATIONS: 1: The Immunopharmacology Research Group, Medical School, Tampere University Hospital, Tampere, Finland; Research Unit, Tampere University Hospital, Tampere, Finland 2: Department of Pharmacology and Therapeutics, Institute of Infection, Immunity and Inflammation, Faculty of Medicine, University of Calgary, Calgary, Alberta, Canada 3: Research Unit, Tampere University Hospital, Tampere, Finland; Department of Respiratory Medicine, Tampere University Hospital, Tampere, Finland The full text article is available for purchase 25.TAX Pressing the buy now button more than once may result in multiple purchases CREDIT/DEBIT CARD INSTITUTIONAL PAYMENT ACCOUNT OR Purchase later Key: - Free Content - New Content - Subscribed Content - Free Trial Content Website (c) 2007 Ingenta.Read more Netrin-1 receptor-deficient mice show enhanced mesocortical dopamine transmission and blunted behavioural responses to amphetamine
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