06232.Abstract A PRE-DOCKING ROLE FOR MICROTUBULES IN INSULIN-STIMULATED GLUCOSE TRANSPORTER 4 TRANSLOCATION Yu Chen, Yan Wang, Wei Ji, Pingyong Xu and Tao Xu National Key Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China T.Or P.Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China Fax: +86 10 64867566 Tel: +86 10 64888469 E-mail: or These authors contributed equally to this workEGFP, enhanced green fluorescence protein; GLUT4, glucose transporter 4; GSV, GLUT4 storage vesicle; PC, percentage colocalization; PM, plasma membrane; TIRFM, total internal reflection fluorescence microscopy.Glucose uptake by inducing translocation of glucose transporter 4 (GLUT4) from intracellular resides to the plasma membrane.GLUT4 storage vesicles are translocated from the cellular interior to the plasma membrane remains to be elucidated.The present study, intracellular transport of GLUT4 storage vesicles and the kinetics of their docking at the plasma membrane wereprehensively investigated at single vesicle level in control and microtubule-disrupted 3T3-L1 adipocytes by time-lapse total internal reflection fluorescence microscopy.Is demonstrated that microtubule disruption substantially inhibited insulin-stimulated GLUT4 translocation.Analysis reveals that microtubule disruption blocked the recruitment of GLUT4 storage vesicles to underneath the plasma membrane and abolished the docking of them at the plasma membrane.Read more [automation and analytical techniques] nanogold catalysis based immunoresonance-scattering spectral assay for trace complement component 3
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